SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Mussatto,Solange Inês; Roberto,Inês Conceição.

Xylitol was produced by Candida guilliermondii from charcoal-treated and untreated rice straw hemicellulosic hydrolysate with or without nutrients (ammonium sulphate, calcium chloride, rice bran extract). Both, xylitol yield and volumetric productivity decreased significantly when the nutrients were added to treated and untreated hydrolysates. In the treated hydrolysate, the efficiency of xylose conversion to xylitol was 79% when the nutrients were omitted. The results demonstrated that rice straw hemicellulosic hydrolysate treated with activated charcoal was a cheap source of xylose and other nutrients for xylitol production by C. guilliermondii. The non-necessity of adding nutrients to the hydrolysate media would be very advantageous since the process...

Evaluation of sorghum straw hemicellulosic hydrolysate for biotechnological production of xylitol by Candida guilliermondii

Palavras-chave: Rice straw hydrolysate; Nutrient; Activated charcoal; Candida guilliermondii; Xylitol.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300020

Sene,L; Arruda,P.V; Oliveira,S.M.M; Felipe,M.G.A.

A preliminary study on xylitol production by Candida guilliermondii in sorghum straw hemicellulosic hydrolysate was performed. Hydrolysate had high xylose content and inhibitors concentrations did not exceed the commonly found values in other hemicellulosic hydrolysates. The highest xylitol yield (0.44 g/g) and productivity (0.19 g/Lh) were verified after 72 hours.

Fractionation of chemical components of oil palm trunk by steam explosion for xylitol and alcohol production

Palavras-chave: Sorghum straw; Hemicellulosic hydrolysate; Xylose; Xylitol; Candida guilliermondii.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300036

Thai Agricultural

Punsuvon, V.; Vaithanomsat, P.; Pumiput, P.; Janthranurak, S.; Anpanurak, W..

Palavras-chave: Chemical; Components; Oil; Palm; Xylitol; Alcohol; Production.

Ano: 2005

URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5023

Genetic analysis of D-xylose metabolism by endophytic yeast strains of Rhodotorula graminis and Rhodotorula mucilaginosa

Genet. Mol. Biol.

Xu,Ping; Bura,Renata; Doty,Sharon L..

Two novel endophytic yeast strains, WP1 and PTD3, isolated from within the stems of poplar (Populus) trees, were genetically characterized with respect to their xylose metabolism genes. These two strains, belonging to the species Rhodotorula graminis and R. mucilaginosa, respectively, utilize both hexose and pentose sugars, including the common plant pentose sugar, D-xylose. The xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) genes were cloned and characterized. The derived amino acid sequences of xylose reductase (XR) and xylose dehydrogenase (XDH) were 32%~41% homologous to those of Pichia stipitis and Candida. spp., two species known to utilize xylose. The derived XR and XDH sequences of WP1 and PTD3 had higher homology (73% and 69% identity)...

Improvement of biotechnological xylitol production by glucose during cultive of Candida guilliermondii in sugarcane bagasse hydrolysate

Palavras-chave: Rhodotorula graminis; Rhodotorula mucilaginosa; D-xylose metabolism; Xylitol; Xylitol dehydrogenase; Xylose reductase.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300018

Silva,Débora Danielle Virgínio da; Mancilha,Ismael Maciel de; Silva,Silvio Silvério da; Felipe,Maria das Graças de Almeida.

The effect of glucose on xylose-to-xylitol bioconversion by Candida guilliermondii was examined by adding it to sugarcane bagasse hydrolysate medium to obtain different glucose:xylose ratios (1:25, 1:12, 1:5 and 1:2.5). Under experimental conditions, increasing glucose:xylose ratio improved the assimilation of the xylose present in the hydrolysate by yeast, resulting in biomass increase, and in the formation of xylitol and glycerol/ethanol by-products. Maximum values of xylitol yield (0.59 g g-1) and volumetric productivity (0.53 g l-1.h-1) were obtained with glucose:xylose ratio of 1:5, resulting in the higher conversion efficiency (64.3%).

In vitro inhibition of adhesion of Escherichia coli strains by Xylitol

Palavras-chave: Candida guilliermondii; Glucose; Sugarcane bagasse hydrolysate; Xylitol; Xylose.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200005

Silva,Annelisa Farah da; Suzuki,Érika Yoko; Ferreira,Aline Siqueira; Oliveira,Murilo Gomes; Silva,Sílvio Silvério da; Raposo,Nádia Rezende Barbosa.

The present study aimed to evaluate xylitol's antimicrobial and anti-adherence activities on Escherichia coli (ATCC 8739) and on another clinical strain enteropathogenic E. coli (EPEC). In vitro minimum inhibitory concentration (MIC) test and adhesion assays were performed using 0.5, 2.5 and 5.0% xylitol. It was found that xylitol did not have antimicrobial properties on these strains. The scanning electron microscopy (SEM) demonstrated that the slides treated with xylitol had a significant reduction in the number of bacilli and the inhibition of microbial adhesion was probably the xylitol's mechanism of action. Xylitol could be a possible alternative on the control of E. coli infections.

In vitro inhibition of Pseudomonas aeruginosa adhesion by Xylitol

Palavras-chave: Bacterial adhesion; Escherichia coli; Scanning electron microscopy; Xylitol.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200003

Sousa,Letícia Pinheiro de; Silva,Annelisa Farah da; Calil,Natalia Oliveira; Oliveira,Murilo Gomes; Silva,Silvio Silvério da; Raposo,Nádia Rezende Barbosa.

This study evaluated, in vitro, the antimicrobial activity and the anti-adherent property of xylitol (0.5, 2.5 and 5.0%, w/v) on two Pseudomonas aeruginosa strains (ATCC 9027 and clinical). The assay of antimicrobial activity was performed to determine a minimum inhibitory concentration (MIC) and the adhesion test was performed, by which the parameters regarding, growth in the culture medium, number of colony forming units (CFUs) released and slide evaluation by scanning electron microscopy (SEM) were analyzed. The Statistical Package for the Social Sciences (SPSS) was employed for statistical analysis. Results showed that xylitol had no antimicrobial activity on these strains; however, the inhibition of bacterial adherence was observed in microphotographs...

Inhibitory effect of acetic acid on bioconversion of xylose in xylitol by Candida guilliermondii in sugarcane bagasse hydrolysate

Palavras-chave: Bacterial adhesion; Pseudomonas aeruginosa; Prevention and control; Xylitol.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500004

Silva,Débora D.V.; Felipe,Maria G.A.; Mancilha,Ismael M.; Luchese,Rosa H.; Silva,Sílvio S..

Sugarcane bagasse hydrolysate (initial acetic acid concentration = 3.5g/L), was used as a fermentation medium for conversion of xylose into xylitol by the yeast Candida guilliermondii FTI 20037. Acetic acid (2.0g/L) was added to the medium at different times of fermentation, with the aim of evaluating its effects on the bioconversion process. The addition of acetic acid to the medium after 12h of fermentation resulted in the strongest inhibition of the yeast metabolism. In this case, the xylose consumption and cell growth were, respectively, 23.22 and 11.24% lower than when acid was added to the medium at the beginning of fermentation. As a consequence of the inhibitory effect, lower values of the xylitol yield (0.39g/g) and productivity (0.22g/L.h) were...

Obtaining partial purified xylose reductase from Candida guilliermondii

Palavras-chave: Xylitol; Xylose; Acetic acid; Sugarcane bagasse hydrolysate.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200014

Tomotani,Ester Junko; Arruda,Priscila Vaz de; Vitolo,Michele; Felipe,Maria das Graças de Almeida.

The enzymatic bioconversion of xylose into xylitol by xylose reductase (XR) is an alternative for chemical and microbiological processes. The partial purified XR was obtained by using the following three procedures: an agarose column, a membrane reactor or an Amicon Ultra-15 50K Centrifugal Filter device at yields of 40%, 7% and 67%, respectively.

Reclassification of Candida guilliermondii FTI 20037 as Candida tropicalis based on molecular phylogenetic analysis

Palavras-chave: Xylose reductase; Xylitol; Partial purification.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300027

Lima,Luanne Helena Augusto; Felipe,Maria das Graças de Almeida; Torres,Fernando Araripe Gonçalves.

Yeasts of the genus Candida are of clinical importance and also have many industrial applications, mainly in the food industry. The yeast Candida guilliermondii FTI 20037 has been extensively studied in order to establish a biotechnological process for the production of xylitol. The goal of this study was to verify the taxonomic classification of this strain based on the analysis of rDNA sequences and the xyl1 gene. DNA fragments from these sequences were amplified by PCR and BLAST analysis revealed strong identity with the corresponding sequences from Candida tropicalis. Based on these results, we propose that C. guilliermondii FTI 20037 must be reclassified as C. tropicalis.

Screening of filamentous fungi for production of xylitol from D-xylose

Palavras-chave: Candida guilliermondii; Candida tropicalis; Ribosomal DNA; Xylitol.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500033

Sampaio,Fábio Coelho; Silveira,Wendel Batista da; Chaves-Alves,Virgínia Maria; Passos,Flávia Maria Lopes; Coelho,Jorge Luiz Cavalcante.

Eleven filamentous fungi were screened for xylitol production in batch cultures. Production was generally low under the growth conditions used in this study. Penicillium crustosum presented the highest production, 0.52 g L-1 from 11.50 g L-1 of D-xylose, representing consumption of 76% of the original D-xylose.

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Xylose; Bioconversion; Xylitol; Filamentous fungi.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400007

Sugarcane straw as a feedstock for xylitol production by Candida guilliermondii FTI 20037

Hernández-Pérez,Andrés Felipe; Arruda,Priscila Vaz de; Felipe,Maria das Graças de Almeida.

Abstract Sugarcane straw has become an available lignocellulosic biomass since the progressive introduction of the non-burning harvest in Brazil. Besides keeping this biomass in the field, it can be used as a feedstock in thermochemical or biochemical conversion processes. This makes feasible its incorporation in a biorefinery, whose economic profitability could be supported by integrated production of low-value biofuels and high-value chemicals, e.g., xylitol, which has important industrial and clinical applications. Herein, biotechnological production of xylitol is presented as a possible route for the valorization of sugarcane straw and its incorporation in a biorefinery. Nutritional supplementation of the sugarcane straw hemicellulosic hydrolyzate as a...

Xylitol inhibits J774A.1 macrophage adhesion in vitro

Palavras-chave: Sugarcane straw; Hemicellulosic hydrolyzate; Xylitol; Nutritional supplementation; Initial oxygen availability.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200489

Ferreira,Aline Siqueira; Souza,Maria Aparecida de; Raposo,Nádia Rezende Barbosa; Ferreira,Ana Paula; Silva,Silvio Silvério da.

The aim of this work was to evaluate the effect of xylitol on J774A.1 macrophage adhesion. Adhesion consisted of a three-hour interval, at room temperature, followed by washing and cell incubation at 37ºC/5% CO2/ 48h. Xylitol was used to treat the cells either before (for 24h) or after the cell incubation (for 48h) at 5% as final concentration in both the situations. It was found that xylitol was effective in preventing the adhesion in both the conditions in spite of the former being 100-fold greater and significant (p < 0.001). The results pointed to an important xylitol action on macrophage adhesion, which should be further investigated as an inflammatory control.

Xylitol production by Candida parapsilosis under fed-batch culture

Palavras-chave: Adhesion; J774A; 1 macrophages; Xylitol.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600017

Furlan,Sandra A.; Castro,Heizir F. de.

Xylitol production by Candida parapsilosis was investigated under fed-batch cultivation, using single (xylose) or mixed (xylose and glucose) sugars as substrates. The presence of glucose in the medium induced the production of ethanol as secondary metabolite and improved specific rates of growth, xylitol formation and substrate consumption. Fractionated supply of the feed medium at constant sugar concentration did not promote any increase on the productivity compared to the single batch cultivation.